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Natura Croatica Issue number: Natura Croatica, Vol. 17 No.4

Elektrokemijsko određivanje metalotioneina kod domaće peradi

Authors: Miroslava Beklova, Ivo Fabrik, Pavlina Sobrova, Vojtech Adam, Jiri Pikula, Rene Kizek

Authors and affiliation

  • Miroslava Beklova Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
  • Ivo Fabrik Department of Chemistry and Biochemistry Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
  • Pavlina Sobrova Department of Chemistry and Biochemistry Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
  • Vojtech Adam Department of Chemistry and Biochemistry; 3Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic
  • Jiri Pikula Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
  • Rene Kizek Department of Chemistry and Biochemistry Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republi

Abstract

Metallothionein (MT) belongs to group of intracellular, low-molecular and cysteine-rich proteins with a molecular weight from 6 to 10 kDa. Owing to their high affinity to heavy metals (Zn, Cd, As, etc.) their main role is homeostatic control and detoxification of metal ions in an organism. In the present work we aimed at suggesting and utilizing electroanalytical techniques to determine content of MT in the blood serum of domestic fowls. Electrochemical measurements were performed with an AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. Particularly, MT was detected by adsorptive transfer stripping technique in connection with differential pulse voltammetry. The detection limit of MT was estimated down to 100 fM (standards only) or down to 100 pM measured in the presence of blood serum. The average content of MT was 21.3 µM. The MT level in hens was about 25 % higher than in cocks. This phenomenon can be related to higher demands on the content of this protein in hens due the requirement for ion transport to form eggshell.

Keywords

electrochemical method, differential pulse voltammetry, Brdicka reaction, protein, bird * Corresponding author's e-mail: kizek@sci.muni.cz, phone: +420-5-4513-3350, fax: +420-5-4521-2044 Croatian Natural History Museum, Demetrova 1, Zagreb, Croatia 284

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