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H. Bilandžija
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Jana Bedek
Croatian Biospeleological Society, Demetrova 1, 10000 Zagreb, Croatia
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Branko Jalžić
Croatian Biospeleological Society (CBSS), Rooseveltov trg 6, 10 000 Zagreb, Croatia
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Sanja Gottstein
Division of Zoology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
Abstract
The distribution and morphological variability of the troglobiotic freshwater sponge Eunapius subterraneus Sket & Velikonja, 1984 registered in Croatia as a threatened species in the IUCN category Endangered (EN) was investigated. The research area encompassed Velika and Mala Kapela Mountains as well as the upper catchment area of the Dobra and Mre`nica rivers on the NE side of Velika and Mala Kapela Mountains, mostly in the Ogulin–Pla{ki karst plateau. The species was registered in six localities, three previously known from the literature and three constituting new records: Mandelaja pit and the caves Crna~ka {pilja and Izvor {pilja Gojak. For subspecies identification the morphology and dimensions of megascleres and gemmuloscleres of the collected samples were analyzed. Significant differences among sponge populations were indicated by one- -way ANOVA (p < 0.001) in width and length of megascleres. The subspecies E. s. mollisparspanis Sket & Velikonja, 1984 was found only at its type locality, in the cave Rudnica {pilja VI. The preliminary variability of other populations after discriminant function analysis of measured morphometric parameters such as the width and length of mega- and gemmulo-scleres indicates four groups, but it was not possible to separate them clearly in all cases. Subterranean sponges were not found in two localities known from the past, the ula – Medvedica cave system and the cave [pilja u kamenolomu Tounj, both under great pressure of groundwater habitat changes because of water pollution, river direction changes and habitat destruction.
Keywords
freshwater sponges, caves, springs, gemmules, spicules, distribution, Croatia Croatian Natural History Museum, Demetrova 1, Zagreb, Croatia 2